PPT Slide
Biotechnology Center for Agriculture and the Environment Rutgers, The State University of New Jersey
1. Acidify 1 liter of groundwater to a pH ɚ with HCl.
2. Transfer to a 2 liter separatory funnel and extract three times with 200 ml of
3. Evaporate the collected ether under vacuum using a rotovap device.
4. After evaporating, rinse the flask twice with 5 ml of clean ether.
5. Transfer the ether into a 10 ml glass pipette filled with anhydrous sodium sulfate
to remove any residual water.
6. Rinse the drying column with 5 ml of clean ether.
7. Collect the ether and dry under a stream of argon gas.
8. Dissolve the sample in 100 µl of methylene chloride.
9. Derivatize with 100 µl of bis(trimethyl-silyl)trifluoro-acetamide.