Hopane is used as an internal marker for measurement of remediation effectiveness

Total oil depletion = (1 –

200 ppm

400 ppm

) x 100 = 50%

Estimation of Total Oil Depletion

100 mg Fresh Oil

Degraded Oil

Hopane

Hopane Concentration in oil = 200 ppm

Hopane Concentration in remainingoil = 400 ppm

Hopane

50 mg

Degraded Oil